Microscopical and Phytochemical Analysis of Fruit, Leaves and Roots of Fragaria nubicola (Lindl.Ex Hook.F.) Lacaita

Background and Objective: Developing countries continue to rely on plant-based remedies for disease treatment and prevention. The standardization of plant material can be checked by using pharmacognostic techniques. The current research work was carried out to investigate the macro, microscopical, and phytochemical features (Qualitatively) of the root, fruit, and leaf of F. nubicola. Materials and Methods: Standard methods were used to conduct the epidermal investigation on the leaf. Protocols were used to screen for qualitative phytochemicals. Different elements were found after a study of the elements was done using the X-ray Fluorescence (XRF) spectroscopy technique. The color, taste, texture, and aroma of leaves, roots, and fruit were revealed by their macroscopic properties. Green color for the leaf, pinkish-red for the fruit, and brownish for the root was observed. The statistical tools and techniques used for analyzing data include descriptive statistics, Chi-square test, frequency analysis, One-way ANOVA, and heatmaps. The standard significant levels were set at p<0.05. Results: Preliminary phytochemical tests of three chemical extracts indicated the presence of carbohydrates, alkaloids, terpenoids, phlobatannins, flavonoids, tannins, anthraquinones, and steroids in leaves. Similarly, substances were discovered in the plant fruit, including sugars, proteins, terpenoids, flavonoids, saponins, anthraquinones, and steroids. Numerous macro and microelements, including P, Zr, Fe, Si, Mn, Nb, Mo, Mg, and S, were found in fruits after an elemental analysis. They are considered to be essential to both the physiological functioning of plants and the effects of crude drugs. Conclusion: This research will provide a novel way for scientists to separate physiologically active components, which may help them find new compounds that can treat a variety of diseases.