Estimation of Lawsone Release from Lawsonia inermis Leaf Powder

Background and Objective: In ancient times people use various colouring materials for the hair to maintain the hair looking young and nourish look. Among the various herbal materials, the henna powder is very much pronounced with traditional users and it delivers an orange colour shade. The present attempt exclusively finds out the optimized time for the henna release through the quantification method and the results are correlated with dye out and dye uptake on the grey swatches. Materials and Methods: Pure henna powder was collected from high crop season in Rajasthan henna growing garden in India and tested the initial lawsone content through HPLC assay. At various time intervals, the lawsone release potential is estimated through the HPLC method, to estimate the lawsone kinetics. Results: The lowest lawsone release was recorded at 30 min and the value was 0.0657% the highest lawsone release was noticed in 4 hrs soaking samples and the lawsone value was 0.3014%. Around 4 hrs reach the maximum lawsone content i.e., 93% recovery noticed. Study results indicated that the maximum lawsone content was released at 4-6 hrs. The dye uptake value (DE) is higher in 4 hrs soaking time i.e., 24.01 unit, around DE value 4 unit lower in immediate and 30 min soaking time. Conclusion: Lawsone release value gradually increased with soaking time and reach the maximum at 4-6 hrs and then gradually decreased. Lawsone released a quantification study that was immensely useful to draw the usage pattern of henna powder for the consumer to follow the correct method to achieve the optimum active potential during the colouring practice and get the vibrant colour delivery.