Metal and Microbial Contamination in the WaterAvailable for Agriculture in Selected FarmSettlements in Nigeria

Background and Objective: Several diseases have been reportedly linked to the consumption of water with metal and/or microbial contamination, or agricultural produce watered by such method. This study assessed the water available for agriculture in three selected farm settlements in Southwestern Nigeria. Materials and Methods: As 15, 12 and 5 water samples from the Ajegunle, Akufo and Eruwa farm settlements, respectively were analyzed for 11 heavy and trace metals and 7 microbial populations, using standard laboratory procedures, for dry and wet seasons. Analysis of Variance (ANOVA) and Duncan’s Multiple Range Tests were done using SPSS, version 20. Pollution sources were apportioned using Principal Component Analysis while seasonal spatial variation maps were generated via the Inverse Density Weighted method. Results: Water from the three farm settlements showed possibilities of cadmium, iron, manganese, E. coli and streptococcal contaminations. The pollution sources identified were bedrock weathering, fertilizer/agricultural waste/run-off leachates, agricultural activities, fecal contamination, livestock agricultural wastes, sewage effluents and organic decomposition. Spatial maps showing the existing distributions of selected metals and microbial populations within Ajegunle and Akufo farm settlements were produced. Conclusion: This research has generated information for the eradication or substantial reduction of the potential harm of metal and microbial contamination to the farm settlers and consumers of their agricultural produce.


INTRODUCTION
Over time, there have been reports of pollution from high levels of heavy and trace metals in different media as well as negative health effects on humans resulting from consumption via the food chain 1 . The use of contaminated water for irrigation and livestock farming has been considered to be responsible for several disease outbreaks after consumption of such produce 2,3 . Hence the need to assess and monitor their concentrations in plant tissues and animal meat to curb their excessive build-up in the human food chain 1,4,5 .
Researchers have used multivariate statistical analyses such as principal component analysis (PCA) and cluster analysis (CA) to analyze water quality and apportion pollution sources [6][7][8][9] . Also, geographic information system (GIS), using different techniques, such as inverse distance weighted (IDW) interpolation, etc. has been used for site suitability analyses and inventory data management, solute transport and leaching studies, assessment of groundwater contamination vulnerability and modeling of groundwater flow as well as spatial mapping by integrating water quality data with spatial data 10,11 . Spatial groundwater variation maps have been generated by Sahoo et al. 12 and Gidey 13 among others.
This study assessed the contamination, apportioned pollution sources using principal component analysis (PCA) and mapped the spatial variabilities of heavy and trace metals as well as microbial populations in the water samples collected from the Ajegunle, Akufo and Eruwa farm settlements.
Despite many years of cultivation and use of fertilizers in these predominantly agricultural farm settlements, information on heavy and trace metal concentrations as well as microbial populations and their potential dangers were not available till the time this research was conducted. The findings from this research will enhance good and systematic utilization of the water within the settlements in such a way as to eradicate, or at least minimize the potentially harmful effects of metal and microbial contamination in plants, animals and eventual human consumers.

MATERIALS AND METHODS Study area:
This study was conducted in three selected farm settlements within the Southwestern part of Nigeria, Ajegunle, Akufo and Eruwa farm settlements (Fig. 1a). Akufo and Eruwa are in Ido and Ibarapa East Local Government of Oyo State, respectively while Ajegunle farm settlement is in the Obafemi Owode Local Government of Ogun State.
The three farm settlements are located within the Ogun River Basin, within Latitudes 6°26'N and 9°10'N and Longitudes 2°28'E and 4°8'E. The basin is of generally low relief with a North-South direction gradient and experiences two seasons: Dry from November to March and wet from April to October 14,15 . The three farm settlements fall within the basement complex terrain ( Fig. 1b and c). The Abeokuta formation and migmatite underlie the Ajegunle farm settlement while Akufo and Eruwa are underlain with quartzite and undifferentiated gneiss, respectively 9,16 .
The Ajegunle farm settlers are majorly livestock farmers in poultry, fish farming and piggery, but they also grow maize and cassava. The Akufo farm settlers grow cassava, yam, oil palm, kola nut, cocoa and timber as well as do poultry farming and cattle rearing. In the Eruwa farm settlement, the prevalent crops are cassava, vegetables, cashew and fruits such as watermelon while they also engage in livestock farming such as piggery. These farm settlements have a long history of over 60 years of cultivation of staple crops and use of pesticides and fertilizers as well as other veterinary drugs and agrochemicals.  The PCA was carried out after the necessary standardization and application of the Kaiser-Mayer-Olkin (KMO) measure of sampling adequacy. Only components with eigenvalues greater than 1.00 were retained as the extracted components [24][25][26][27][28] .

IDW spatial variation mapping:
This was achieved by geo-referencing and digitizing the base maps via the Arc GIS software, version 10 (2015). The inverse distance weighted (IDW) interpolation technique was used to generate and integrate spatial and attribute databases. Spatial variation maps of the heavy and trace metals, as well as the microbial populations, were then generated for the wet and dry seasons to show the patterns and seasonal variabilities within Ajegunle and Akufo farm settlements 29,30 . The sampling points within the Eruwa farm settlement were very few and very wide apart, hence spatial variation maps were not generated for the settlement using IDW.

Means and standard deviations:
The means and standard deviations, minimum and maximum values for heavy and trace metal concentrations (mg LG 1 ) and microbial populations (×10 5 CFU mLG 1 ) in the water samples collected within Ajegunle farm settlement are presented in Table 1.
The means and standard deviations, minimum and maximum values for heavy and trace metal concentrations (mg LG 1 ) and microbial populations (×10 5 CFU mLG 1 ) in the water samples collected within the Akufo farm settlement are presented in Table 2.
The means and standard deviations, minimum and maximum values for heavy and trace metal concentrations (mg LG 1 ) and microbial populations (x10 5 CFU mLG 1 ) in the water samples collected in the Eruwa farm settlement are presented in Table 3.  The result of the principal component analysis of heavy and trace metals in the water samples collected from the Akufo farm settlement showed 2 extracted components while that of microbial populations showed only 1 extracted component ( Table 5).

Principal component analysis
The result of the principal component analysis of heavy and trace metals in the water samples collected from the Eruwa farm settlement revealed only 1 extracted component while that of microbial populations showed 2 extracted components ( Table 6).

DISCUSSION
For the water samples collected from the Eruwa farm settlement, the high levels of Copper in the wet season were likely due to agricultural activities. As shown via this study, the groundwater from the three farm settlements should be monitored and treated for cadmium, iron and manganese contaminations.
Heavy metals are usually either dissolved in water or occur as colloids and/or particles 31 . They occur in water bodies naturally as eroded minerals within sediments, leached ore deposits, etc., or originate from anthropogenic sources such as solid waste disposal, as well as industrial, agricultural and domestic effluents 32 . Dissociations from bedrocks through which groundwater flows also contribute to metal concentrations in it 33 . Levels of Copper above 1 mg LG 1 in water have been noted to tend to cause astringent tastes 34 .
As revealed via this study, Arsenic poses no threat to the water within the three farm settlements.
Kayode et al. 35  to high blood pressure and kidney damage as well as the destruction of red blood cells and testicular tissues. Cadmium, due to their chemical similarity, may replace Zinc in some enzymes and thus alter the stereo-structure of the enzymes, impairing their catalytic activities 35,38 .
The major sources of Manganese are ores, rocks, fertilizers, steel production, pesticides and battery charging 34 . Studies have associated cognitive behavioral problems in children with Manganese concentration in drinking water 39,40 .
Iron is common in rural groundwater supplies with concentration levels between 0 and 50 mg LG 1 .
According to Al Maliki et al. 37 , it is not toxic to plants where the soil is aerated but can enhance processes that lead to soil acidification and loss of phosphorus, which is an important element for plant well-being.
Iron can accumulate on plant leaves and/or fruits as a result of long-term irrigation with water containing Iron in high concentrations, which in turn can reduce the quality of crop production 37,41 . No serious health implications have been reported in humans 42 . It has however been noted that in high concentrations, Iron may produce adverse neurological effects 43 . Iron-mediated oxidative damage to the mitochondrial genome may result from long-term iron contamination, eventually adversely affecting functionality 40,44 .
The concentrations of Iron and Manganese in this study follow a similar trend to what Emenike et al. 45 observed, Iron and Manganese usually exist together in water, the concentration of Iron being always higher than that of Manganese because it is more abundant in the earth crust.
As excess heavy and trace metal intake by humans through the food chain (or agriculture) has been reported to be potentially dangerous in many countries 1 , it is recommended that the water from the three farm settlements be monitored and treated for cadmium, iron and manganese contaminations. This is to protect the consumers of agricultural produce from the potential dangers of consuming the metals in higher quantities than permissible. Also, crops that are resistant to, or require high concentrations of these metals are recommended for cultivation within the farm settlements.
Several studies have shown that microbial pathogens, such as Salmonella, E. coli, S. faecalis and enteroviruses are relatively stable in underground water [46][47][48][49] , which is a major source of water for agriculture within the farm settlements. High coliform counts appear to be characteristic of rural groundwater quality in Nigeria 50 . Meanwhile for the three farm settlements, coliform counts for both dry and wet seasons considered were revealed to be safe. However, E. coli and Streptococcal counts for the two seasons suggest the need for appropriate treatment before use. This likely resulted mostly from livestock wastes in the farm settlements, since livestock agriculture is a major occupation of the settlers. It could as well be suspected that these contaminants infiltrated from human and animal wastes into the hand-dug wells 51 . Escherichia coli is regarded as the most sensitive indicator of faecal pollution and its presence in a water sample is a major health concern which calls for remedial attention 51 . Its presence also indicates that other enteric pathogens may be present 52 . Streptococcus faecalis, Staphylococcus aureus and Bacillus sp., have been implicated to be possibly responsible for gastro-intestinal disorders 53 . Salmonella and vibrio cholera were not found in any of the samples examined from the farm settlements.
Consumption of contaminated water has been known to result in diseases such as diarrhea, meningitis, acute renal failure, urinary tract infections and haemolytic anaemia, or indirectly from contaminated agricultural produce. Meanwhile, Terzieva and McFeters 54 as well as Pandey et al. 49 noted that controlling pathogen contamination from livestock/wildlife can be challenging.
The principal component analysis (PCA) is a multivariate statistical technique that can use mutual correlation coefficients to relate variables to principal components or factors. When used in hydrochemistry, these may be interpreted based on specific or multiple hydrochemical processes like mineralization, lithology and environmental processes 8,55 .
The PCA extracted 4 components for Ajegunle farm settlement. The PC 1 resulted very likely from the weathering of bedrock materials, in line with Kwami et al. 56 . The PC 2 was likely from the leaching of agricultural wastes and/or chemicals. The PC 3 was likely due to leachate from fertilizers/agricultural wastes or run-off while PC 4 resulted from agricultural activities. Fertilizers used for agriculture are wellknown sources of cadmium and copper 57 . For the Akufo farm settlement, PCA produced 2 components. The PC 1 resulted very likely from weathering of bedrock materials, similar to apportionment made by Shrestha et al. 58 , while PC 2 was likely due to leachate from fertilizers/agricultural wastes or run-off 57,59 .
Meanwhile, for the Eruwa farm settlement, the only PC extracted was proposed to have been from weathering of bedrock materials and leachate from fertilizers/agricultural wastes or run-off, in line with the findings of Elumalai et al. 57 The farm settlements have a long history of over 60 years of cultivation of staple crops, use of pesticides, fertilizers, other veterinary drugs and agrochemicals.
Heavy and trace metal concentrations/distributions have been reported to vary seasonally 60,61 . This study revealed higher concentrations of most of the metals during the wet season than during the dry. Agricultural activities form a major source of metal and microbial contamination in the water within the three farm settlements, as apportioned earlier via PCA. It is also the predominant occupation of the settlers and due to water availability challenges during the dry season, they depend largely on rainfall and availability of more water during the wet season. Hence, the higher concentrations of the metals during the wet season were very likely because, within the farm settlements, agricultural activities increased during the wet season.
For microbial populations, 2 components were extracted via PCA for the Ajegunle farm settlement. The PC 1 was a result of fecal contamination 59  It is hereby recommended that routine groundwater quality assessment be carried out to follow the dynamics and be able to manage and control pollution within the farm settlements. Also, appropriate treatments should be administered to the groundwater, especially, to take care of magnesium, iron, cadmium, manganese, E. coli and Streptococcal contaminations. Since, this study identified high magnesium concentrations in the study areas, magnesium-tolerant crops will thrive better. Finally, effective and sustainable agricultural (especially livestock) waste management strategies as well as pollution control against agricultural run-off and chemicals are necessary within the farm settlements to curb contamination and ensure good health for the settlers.
A limitation of this study is that the water sources within the Eruwa farm settlement were very few and wide apart and hence spatial maps could not be generated for the settlement.

CONCLUSION
The water samples from the three farm settlements showed possibilities of cadmium, iron, manganese Escherichia coli and streptococcal contaminations and therefore should be monitored and treated accordingly before use for agricultural or other purposes. Also, crops that are resistant to, or require high concentrations of cadmium, iron and manganese are recommended for cultivation within the farm settlements. The information from this research will help safeguard the lives of the farm settlers as well as the consumers of agricultural produce from the settlements against metal and microbial contamination.

SIGNIFICANCE STATEMENT
This study assessed the contamination, apportioned pollution sources using principal component analysis consumers of agricultural produce from the settlements against metal and microbial contamination. The water samples from the three farm settlements showed possibilities of cadmium, iron, manganese, Escherichia coli and streptococcal contaminations and therefore should be monitored and treated accordingly before use for agricultural or other purposes.